Journal: MedComm

Article Title: Comprehensive Integrated Single‐Cell and Spatial Transcriptomics Unveil the Dynamic Landscape of Betel Nut‐Associated Oral Mucosal Carcinogenesis and Its Tumor Microenvironment

doi: 10.1002/mco2.70796

Figure Lengend Snippet: Arecoline enhances the migratory and invasive potential of LAMC2 + OSCC cells. (A) Representative immunohistochemical staining images showing LAMC2 expression in OSCC tissues from betel nut chewers and nonchewers. The box‐and‐whisker plot on the right summarizes LAMC2 expression levels; each dot represents an individual clinical specimen (**** p < 0.0001 by Student's t ‐test). (B) Western blot images showing the expression of LAMC2, EMT‐associated markers (N‐cadherin, E‐cadherin, and Vimentin), and GAPDH in OSCC cell lines SCC25 and SCC9 under control and arecoline‐treated conditions. (C) Representative Transwell assay images illustrating migratory and invasive capacities of SCC25 and SCC9 cells in control and arecoline‐treated groups. (D) Bar plots showing the quantification of migration and invasion assays for SCC25 and SCC9 cells under control and arecoline‐treated conditions ( n = 3, *** p < 0.001, **** p < 0.0001 by Student's t ‐test).

Article Snippet: SCC9 and SCC25 cells were obtained from the American Type Culture Collection.

Techniques: Immunohistochemical staining, Staining, Expressing, Whisker Assay, Western Blot, Control, Transwell Assay, Migration

Journal: Frontiers in Immunology

Article Title: Evaluation of a multi-component mucoadhesive buccal patch: cytokine-associated inflammatory responses and tissue remodeling in experimental models

doi: 10.3389/fimmu.2026.1847576

Figure Lengend Snippet: In vitro cytotoxic and functional effects of the IBL patch in SCC9 oral cancer cells. (A) MTT assay showing dose-dependent reduction in SCC9 cell viability following treatment with increasing concentrations of the IBL patch and individual bioactives. (B) Colony formation assay demonstrating decreased clonogenic survival in treated SCC9 cells. (C) Wound-healing assay illustrating impaired migratory capacity of SCC9 cells at 0, 12, and 24 h following treatment. (D) AO/EtBr dual staining indicating dose-dependent induction of apoptosis in SCC9 cells, with representative fluorescence images and quantitative analysis of live and apoptotic populations. Data are presented as mean ± SD (n = 3). Statistical significance was determined relative to untreated controls (*P < 0.05, **P < 0.01, ***P < 0.001 vs Control).

Article Snippet: SCC9 cells obtained from ATCC were culture in DMEM/F12 with 10% FBS and 1% Penstrep and McCoy, KB-3–1 cell lines (NCCS, Pune, India) were cultured in DMEM with 10% FBS and 1% Penstrep and.

Techniques: In Vitro, Functional Assay, MTT Assay, Colony Assay, Wound Healing Assay, Staining, Fluorescence, Control

Journal: Frontiers in Immunology

Article Title: Evaluation of a multi-component mucoadhesive buccal patch: cytokine-associated inflammatory responses and tissue remodeling in experimental models

doi: 10.3389/fimmu.2026.1847576

Figure Lengend Snippet: IBL patch attenuates inflammatory responses and modulates epithelial markers in SCC9 cells. (A–E) Relative mRNA expression of MMP-2, IL-6, IL-2, TNF-α, and TGF-β under indicated treatment conditions, analyzed by qRT-PCR and normalized to an internal control (2^–ΔΔCt method). Data are presented as mean ± SD (n = 3). (F) Representative Western blots of CK17, CK18, and COX-2 protein expression; GAPDH served as loading control (n = 3). (G) Densitometric quantification of protein levels normalized to GAPDH (mean ± SD, n = 3). (H–J) Immunofluorescence images showing CK17 and COX-2 (green) with DAPI nuclear staining (blue), and corresponding fluorescence intensity quantification under Control, PWD, and IBL-Patch treatments. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant.

Article Snippet: SCC9 cells obtained from ATCC were culture in DMEM/F12 with 10% FBS and 1% Penstrep and McCoy, KB-3–1 cell lines (NCCS, Pune, India) were cultured in DMEM with 10% FBS and 1% Penstrep and.

Techniques: Expressing, Quantitative RT-PCR, Control, Western Blot, Immunofluorescence, Staining, Fluorescence